CRISPR-Cas9How targeted gene editing works, and what it can and cannot do.BiologyAdapted from a bacterial immune system.1Design a guide RNA20 bases complementary to the target, next to a PAM sequence.2Cas9 binds and cutsA double-strand break at the target site.3The cell repairs itNon-homologous end joining is error-prone — this knocks thegene out.4Or supply a templateHomology-directed repair inserts a specified sequence.LIMITSOff-target cuts elsewhere in the genomeHDR is inefficient in non-dividing cellsDelivery into the right tissue is the main obstacleGermline editing raises unresolved ethical questionsThe 2020 Nobel Prize in Chemistry went to Charpentier and Doudna fordeveloping it as a genome-editing tool.CRISPR-Cas9learnposters.com
CRISPR-Cas9 — printable life science wall chart from LearnPosters. Free vector PDF, US Letter and A4.

CRISPR-Cas9, step by step

Adapted from a bacterial immune system.

  1. Design a guide RNA20 bases complementary to the target, next to a PAM sequence.
  2. Cas9 binds and cutsA double-strand break at the target site.
  3. The cell repairs itNon-homologous end joining is error-prone — this knocks the gene out.
  4. Or supply a templateHomology-directed repair inserts a specified sequence.

Limits

  • Off-target cuts elsewhere in the genome
  • HDR is inefficient in non-dividing cells
  • Delivery into the right tissue is the main obstacle
  • Germline editing raises unresolved ethical questions

The 2020 Nobel Prize in Chemistry went to Charpentier and Doudna for developing it as a genome-editing tool.

Questions about the CRISPR-Cas9 poster

What’s on the CRISPR-Cas9 poster?
4 numbered steps, with a full worked run-through. Adapted from a bacterial immune system. Design a guide RNA — 20 bases complementary to the target, next to a PAM sequence.; Cas9 binds and cuts — A double-strand break at the target site.; The cell repairs it — Non-homologous end joining is error-prone — this knocks the gen…; Or supply a template — Homology-directed repair inserts a specified sequence.. The 2020 Nobel Prize in Chemistry went to Charpentier and Doudna for developing it as a genome-editing tool.
Who is the CRISPR-Cas9 poster for?
CRISPR-Cas9 belongs to the Biology section rather than to a school year, because life science is not something one grade owns. Anyone learning genetics can pin it up — a beginner, a student mid-course, or someone revising years later.
When should you use the CRISPR-Cas9 poster?
A guide RNA finds the sequence; Cas9 cuts it. The cell's own repair does the editing. A wall chart earns its place by being glanceable from where the work is happening, so CRISPR-Cas9 belongs on the wall where that life science work actually happens, within glancing distance, rather than filed away.
What other posters go with CRISPR-Cas9?
DNA Sequencing, Epigenetics and Gel Electrophoresis sit alongside CRISPR-Cas9 in the Biology section. Printed together they make a wall rather than a single sheet, which is how a reference set actually gets used.DNA SequencingEpigeneticsGel Electrophoresis
Is the CRISPR-Cas9 poster free to download and print?
Yes. CRISPR-Cas9 downloads as a free PDF with no account, no email and no watermark, like everything else in the Biology section. Print as many copies as you like for a home, a classroom, a library or a tutoring group; reselling the file is the only thing the licence rules out.Read the licence
What size does the CRISPR-Cas9 poster print at?
CRISPR-Cas9 is a vector PDF laid out for US Letter, and prints on A4 with Fit to page — the same file, no separate download. Because every mark on it is drawn rather than photographed, it stays sharp enlarged to A3, A2 or A1 at a copy shop. Colour carries emphasis only, so a greyscale print of CRISPR-Cas9 loses nothing.Printing guide

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